IonOptix MultiCell: High-Throughput E-C Coupling Investigations

To date, high-throughput assessment of real-time isolated cardiomyocyte calcium and contractility has been unavailable to investigators seeking to improve throughput of isolated cells. In particular, the acute contractile effects of beta-adrenergic stimulants limit the ability of investigators to generate substantial paired cellular data sets. Beta-adrenergic stimulation accelerates cardiomyocyte excitation-contraction coupling kinetics through multiple pathways and can act as an experimental measure of cardiomyocyte functional reserve. Here we highlight a novel modality for high-throughput, repeated measures acquisition of isolated cardiomyocyte calcium and contractility prior to and following treatment with the beta adrenergic agonist isoproterenol.